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Role of major histocompatibility complex II antigen‐presentation pathway genes in orange‐spotted grouper infected with Cryptocaryon irritans
Author(s) -
Deng JunJin,
Xu Shun,
Li YanWei,
Xu DongDong,
Mo ZeQuan,
Li JiaZhou,
Dan XueMing,
Luo XiaoChun
Publication year - 2020
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.13256
Subject(s) - biology , major histocompatibility complex , antigen , antigen presentation , immune system , spleen , immunology , antigen processing , virology , mhc class i , t cell
Cryptocaryon irritans , a pathogen model for fish mucosal immunity, causes skin mucosal and systematic humoral immune response. Where and how MHC II antigen presentation occurs in fish infected with C . irritans remain unknown. In this study, the full‐length cDNA of the grouper cysteine protease CTSS was cloned. The expression distributions of six genes (CTSB, CTSL, CTSS, GILT, MHC IIA and MHC IIB) involved in MHC II antigen presentation pathway were tested. These genes were highly expressed in systematic immune tissues and skin and gill mucosal‐associated immune tissues. All six genes were upregulated in skin at most time points. Five genes expected CTSS was upregulated in spleen at most time points. CTSB, CTSL and MHC IIA were upregulated in the gill and head kidney at some time points. These results indicate that the presentation of MHC II antigen intensively occurred in local infected skin and gill. Spleen, not head kidney, had the most extensive systematic antigen presentation. In skin, six genes most likely peaked at day 2, earlier than in spleen (5–7 days), marking an earlier skin antibody peak than any recorded in serum previously. This significant and earlier mucosal antigen presentation indicates that specific immune response occurs in local mucosal tissues.