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Analysis of virulence and immunogenic factors in Aeromonas hydrophila : Towards the development of live vaccines
Author(s) -
Zhao Xianliang,
Wu Gan,
Chen He,
Li Li,
Kong Xianghui
Publication year - 2020
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.13174
Subject(s) - aeromonas hydrophila , biology , virulence , immunogenicity , microbiology and biotechnology , inactivated vaccine , immune system , cyprinus , genotype , dna vaccination , virology , gene , bacteria , immunization , immunology , fish <actinopterygii> , genetics , fishery
Abstract Aeromonas hydrophila , a bacterium that is widespread in aquatic environments, is responsible for causing haemorrhagic disease in both aquatic and terrestrial species. With the purpose of developing a live vaccine, herein we have investigated nine strains of A. hydrophila (Ah‐01 to Ah‐09) isolated from diseased fish. A study of virulence factors that contribute to pathogenicity and immunogenicity in the host Cyprinus carpio suggests that the presence of β‐hly , act and fla genes contribute to pathogenesis: strains Ah‐01, Ah‐02 and Ah‐03 ( β‐hly + / act + / fla + genotype) were highly pathogenic to C. carpio , whereas Ah‐05 and Ah‐06 ( β‐hly − / act − / fla − genotype) showed weak pathogenicity. Accordingly, Ah‐02 and Ah‐03 were selected to prepare inactivated vaccines, whereas Ah‐05 and Ah‐06 were chosen as live vaccines. Ah‐06 live vaccine was found to have the best protective efficacy, with a protective rate of about 85%, whereas rates of other vaccines were significantly lower, in the range 37%–59%. In addition, DNA vaccines based on genes altA , aha and omp showed immune protection rates of 25%, 37.5% and 75%, respectively. Our data demonstrate that the β‐hly − / act − / fla − / altA + / aha + / omp + genotype has weak pathogenicity and high immunogenicity, and provide a simple and effective way to screen for live A. hydrophila vaccines.