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Molecular characterization of three caspases from Bostrychus sinensis and their transcriptional responses to bacteria and viruses
Author(s) -
Ding Yuehan,
Wei Ke,
Yang Xiao,
Jing Fei,
Shen Bin,
Zhang Jianshe
Publication year - 2020
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.13140
Subject(s) - biology , caspase , proteases , apoptosis , peripheral blood mononuclear cell , downregulation and upregulation , spleen , vibrio parahaemolyticus , microbiology and biotechnology , extracellular , immunology , bacteria , gene , in vitro , enzyme , programmed cell death , biochemistry , genetics
The caspase family proteins are aspartate‐specific cysteine proteases that transmit extracellular signals to cells, ultimately cause apoptosis and therefore play a key role in cellular immunity. In this study, we cloned and characterized three caspases from Chinese black sleeper ( Bostrychus sinensis ), Bscasp‐1 , Bscasp‐8 and Bscasp‐9 . Real‐time PCR analysis showed that Bscasp‐1 , Bscasp‐8 and Bscasp‐9 were universally expressed in all tested tissues of B. sinensis. Expression analyses showed that after poly(I:C) stimulation and bacterial ( Vibrio parahaemolyticus ) infection, the three caspases were significantly upregulated. After poly(I:C) stimulation, the change of Bscasp‐1 expression in the head kidney was the most obvious; peak expression was about 80.78‐fold more than that of the control. In addition, the expression of Bscasp‐8 and Bscasp‐9 in the peripheral blood and liver was 167.99‐ and 17.98‐fold higher than that in the control group, respectively. After V. parahaemolyticus infection, the expression peaks of Bscasp‐1 and Bscasp‐8 in the peripheral blood and spleen were 85.82‐fold and 280.83‐fold that of the control. However, the expression of Bscasp‐9 in the peripheral blood was upregulated only 8.33‐fold higher than that in the control group. These results indicate that Bscasp‐1 , Bscasp‐8 and Bscasp‐9 are likely involved in response to viral and bacterial infection.