Saprolegnia molecular phylogeny among farmed teleosts in Nova Scotia, Canada

To identify the pathogens causing saprolegniosis among farmed fish in Nova Scotia, 172 infected tissues and 23 water samples were collected from six species of teleosts: Atlantic salmon ( Salmo salar ), brown trout ( Salmo trutta ), Arctic charr ( Salvelinus alpinus ), brook trout ( Salvelinus fontinalis ), striped bass ( Morone saxatilis ) and rainbow trout ( Oncorhynchus mykiss ) at nine facilities over a 600 km range. Following laboratory culture, 132 isolates were recovered. Six species of oomycetes were identified from analysis of the internal transcribed spacer (ITS) sequence of the nrDNA: Saprolegnia parasitica , Saprolegnia ferax , Saprolegnia diclina , Saprolegnia aenigmatica , Saprolegnia torulosa , Saprolegnia sp. and Pythiopsis cymosa . Further phylogenetic analyses of the ITS and cytochrome c oxidase subunit 1 ( Cox1 ) regions revealed four strains of Saprolegnia parasitica (named here as S1, S2, S3 and S4), of which S1 and S2 were common (37% and 42% of the isolates), and two strains of S. ferax . Among S. parasitica , S2 and S3 are more closely related to each other than to S1 based on the phylogenetic analyses and predicted RNA secondary structure of the ITS region. Sexual structures with a similar morphology were formed by S1 and S3 in vitro , but were not formed by S2.