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Evaluation of zebrafish ( Danio rerio ) as an animal model for the viral infections of fish
Author(s) -
Rakus Krzysztof,
Adamek Mikołaj,
Mojżesz Miriam,
Podlasz Piotr,
ChmielewskaKrzesińska Małgorzata,
Duk Karolina,
KasicaJarosz Natalia,
Kłak Katarzyna,
Rakers Sebastian,
Way Keith,
Steinhagen Dieter,
Chadzińska Magdalena
Publication year - 2019
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12994
Subject(s) - zebrafish , danio , biology , model organism , pathogen , virology , virus , in vivo , microbiology and biotechnology , gene , genetics
Zebrafish ( Danio rerio ) is a laboratory model organism used in different areas of biological research including studies of immune response and host–pathogen interactions. Thanks to many biological tools available, zebrafish becomes also an important model in aquaculture research since several fish viral infection models have been developed for zebrafish. Here, we have evaluated the possible use of zebrafish to study infections with fish viruses that have not yet been tested on this model organism. In vitro studies demonstrated that chum salmon reovirus (CSV; aquareovirus A) and two alloherpesviruses cyprinid herpesvirus 1 (CyHV‐1) and cyprinid herpesvirus 3 (CyHV‐3) are able to replicate in zebrafish cell lines ZF4 and SJD.1. Moreover, CSV induced a clear cytopathic effect and up‐regulated the expression of antiviral genes vig‐1 and mxa in both cell lines. In vivo studies demonstrated that both CSV and CyHV‐3 induce up‐regulation of vig‐1 and mxa expression in kidney and spleen of adult zebrafish after infection by i.p. injection but not in larvae after infection by immersion. CyHV‐3 is eliminated quickly from fish; therefore, virus clearing process could be evaluated, and in CSV‐infected fish, a prolonged confrontation of the host with the pathogen could be studied.