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The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses
Author(s) -
Huang Lixing,
Guo Lina,
Xu Xiaojin,
Qin Yingxue,
Zhao Lingmin,
Su Yongquan,
Yan Qingpi
Publication year - 2019
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12972
Subject(s) - rpos , vibrio alginolyticus , virulence , biology , microbiology and biotechnology , biofilm , bacteria , vibrio , gene expression , gene , genetics , promoter
Vibrio alginolyticus is a leading aquatic pathogen, causing huge losses to aquaculture. rpoS has been proven to play a variety of important roles in stress response and virulence in several bacteria. In our previous study, upon treatment with Cu 2+ , Pb 2+ , Hg 2+ and low pH , the expression levels of rpoS were downregulated as assessed by RNA ‐seq, while impaired adhesion ability was observed, indicating that rpoS might play roles in the regulation of adhesion. In the present study, the RNA i technology was used to knockdown rpoS in V. alginolyticus . In comparison with wild‐type V. alginolyticus , RNA i‐treated bacteria showed significantly impaired abilities of adhesion, growth, haemolytic, biofilm production, movement and virulence. Meanwhile, alterations of temperature, salinity, pH and starvation starkly affected rpoS expression. The present data suggested that rpoS is a critical regulator of virulence in V. alginolyticus ; in addition, rpoS regulates bacterial adhesion in response to temperature, pH and nutrient content changes. These are helpful to explore its pathogenic mechanism and provide reference for disease control.