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Genotypic diversity among Shewanella spp. collected from freshwater fish
Author(s) -
Paździor Ewa,
PękalaSafińska Agnieszka,
Wasyl Dariusz
Publication year - 2019
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12971
Subject(s) - biology , 16s ribosomal rna , shewanella , pulsed field gel electrophoresis , microbiology and biotechnology , shewanella putrefaciens , ribosomal rna , genotype , typing , shewanella oneidensis , bacteria , gene , genetics
Different Shewanella species are isolated both from healthy and from diseased fish. To date, contemporary methods do not provide sufficient insight to determine species and detail differentiation between tested strains. Bacteria isolated from cultured ( n = 33), wild ( n = 12) and ornamental ( n = 6) fish, as well as several reference strains, were tested by 16S rRNA gene sequencing, ERIC‐PCR and pulsed‐field gel electrophoresis (PFGE) assays. Our study indicates that isolates collected from freshwater fish were genetically diverse. Based on 16S rRNA gene sequences, bacteria were clustered into groups S. putrefaciens , S. xiamenensis and S. oneidensis . Some isolates were classified only to genus Shewanella ; thus, 16S rRNA gene analyses were not enough to determine the species. ERIC‐PCR revealed 49 different genotype profiles indicating that the method might be useful for differentiation of Shewanella isolates irrespectively to species identification, contrary to PFGE which is not suitable for Shewanella typing.