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Identification and typing of Vagococcus salmoninarum using genomic and proteomic techniques
Author(s) -
TorresCorral Yolanda,
Santos Ysabel
Publication year - 2019
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12967
Subject(s) - biology , microbiology and biotechnology , typing , serotype , serology , antigen , streptococcus agalactiae , streptococcus pneumoniae , streptococcus , genetics , bacteria , antibiotics , antibody
This study reports on the characterization of Vagococcus salmoninarum using phenotypic, serological, antigenic, genetic and proteomic methods. All strains of V. salmoninarum were resistant to most of the antimicrobials tested, and only 10% of strains were sensitive to florfenicol. Serological analysis demonstrated a high antigenic homogeneity within the species. No cross‐reaction was detected with other fish pathogenic species causing streptococcosis ( Lactococcus garvieae , Streptococcus parauberis , Streptococcus ini ae, Streptococcus agalactiae , Carnobacterium maltaromaticum ) using serum against V. salmoninarum CECT 5810. Electrophoretic analysis of cell surface proteins and immunoblot supported the antigenic homogeneity within V. salmoninarum strains. Moreover, limited diversity was detected using genomic (RAPD, ERIC‐PCR and REP‐PCR) and MALDI‐TOF‐MS analyses. The phenotypic, genomic and proteomic methods tested allowed the rapid differentiation of V. salmoninarum from the other species causing streptococcosis. However, MALDI‐TOF‐MS is the most promising method for typing and characterization of V. salmoninarum .