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Molecular characterization and interactome analysis of aerolysin ( aer ) gene from fish pathogen Aeromonas veronii : The pathogenicity inferred from sequence divergence and linked to histidine kinase ( che A)
Author(s) -
Foysal Md Javed,
Momtaz Farhana,
Ali Md Hazrat,
Siddik Muhammad A. B.,
Chaklader Md Reaz,
Rahman Md Mahbubur,
Prodhan Md Shamsul Haque,
Cole Anthony
Publication year - 2019
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12954
Subject(s) - aeromonas veronii , biology , aerolysin , virulence , sequence analysis , pathogen , gene , carp , microbiology and biotechnology , catfish , common carp , genetics , zebrafish , aeromonas , cyprinus , fish <actinopterygii> , bacteria , fishery
Aerolysin ( aer ) is one of the most important and abundant virulence factors in the infection of fish by Aeromonas veronii . A comprehensive study on the molecular characterization and pathogenicity of the aer gene from 34 A. veronii isolates from diseased carp and catfish was carried out and its interactome was analysed to observe the functional correlations between aer and other proteins within the A. veronii network. The PCR‐based amplification of aer from the 34 isolates of A. veronii showed more aer ‐positive isolates from catfish with a high pathogenic potential in the in vivo challenge test than the carp fish. The analysis of aer gene sequence from challenged fish revealed significant sequence divergence according to the types and geographical distribution of the fish. The networking analysis of aer from the model A. veronii B565 revealed histidine kinase ( che A) as the most functional interacting partner. The study of the interaction between aer from the experimental A. veronii and che A demonstrated that the A chain of che A plays a more important role than the corresponding B chain during contact, and a linker sequence of 15 residues controlled the entire interaction process. Therefore, che A could be an excellent drug target for controlling A. veronii infection of fish.