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Identification of novel sRNAs involved in oxidative stress response in the fish pathogen Vibrio alginolyticus by transcriptome analysis
Author(s) -
Peng Tao,
Kan Jie,
Lun Jingsheng,
Hu Zhong
Publication year - 2019
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12926
Subject(s) - vibrio alginolyticus , oxidative stress , biology , transcriptome , pathogen , catalase , gene , microbiology and biotechnology , virulence , vibrio , gene expression , genetics , bacteria , biochemistry
Vibrio alginolyticus as an important pathogen in aquaculture can encounter the oxidative stress produced by the immune system during infection. Previous studies showed that sRNAs have important functions in response to oxidative stress in bacteria; however, less of sRNAs related to oxidative stress response were identified in V. alginolyticus . In this study, a total of 749 novel sRNAs were identified by RNA sequencing; among them, 128 sRNAs were up‐ or downregulated in response to oxidative stress. In addition, 1,870 genes exhibited variation on mRNA levels in oxidative stress response. By analysing the target genes of the sRNAs, we concluded that these sRNAs could regulate expressions of genes responsible for iron transport, catalase, GSH‐dependent defence system, electron transferred and stress response. Moreover, the functions of the sRNAs are also seemed related to the pathogenicity in V. alginolyticus . Based on the results, we constructed the oxidative stress model in V. alginolyticus . This study provides us the first outlook of sRNAs function in oxidative stress response in V. alginolyticus . Furthermore, this study can help us to prevent and control this important opportunistic pathogen in aquaculture.