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Nocardia seriolae infection in the cultured eel Anguilla japonica in Korea
Author(s) -
Kim Jin Do,
Lee NamSil,
Do Jeung Wan,
Kim Myoung Sug,
Seo Han Gill,
Cho Miyoung,
Jung Sung Hee,
Han HyunJa
Publication year - 2018
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12881
Subject(s) - biology , nocardia , microbiology and biotechnology , 16s ribosomal rna , aquaculture , japonica , strain (injury) , bacteria , necrosis , fish farming , fish <actinopterygii> , veterinary medicine , anatomy , fishery , botany , genetics , medicine
Mass mortality occurred at an Anguilla japonica eel farm equipped with a recirculating aquaculture system in Gimcheon, Korea, from late spring to early summer 2015. The cumulative 3‐month mortality was 16% (approximately 24,300–150,000 fish). The majority of affected fish displayed ulcerative lesions that progressed to petechial haemorrhages and small white granulomas in the major organs. A Gram‐positive, acid‐fast, nonmotile bacterium was isolated from internal organ lesions. Phylogenetic analysis of 16S rRNA identified the species as Nocardia seriolae and the strain was designated EM 150506. Afterwards, naïve eels were injected with 1.8 × 10 7 colony‐forming units per fish to confirm the strain's pathogenicity, which resulted in a 20% mortality rate within 4 weeks. However, surviving fish still exhibited white N. seriolae colonies in internal organs. To our knowledge, this is the first report of a N. seriolae infection in cultured eel.