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Development, characterization and virus susceptibility of a continuous cell line from the caudal fin of marbled eel ( Anguilla marmorata )
Author(s) -
Pao H Y,
Wu C Y,
Huang C H,
Wen C M
Publication year - 2018
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12816
Subject(s) - biology , marbled meat , mesenchyme , virus , cell culture , zoology , microbiology and biotechnology , virology , mesenchymal stem cell , genetics
A continuous cell line consisting mostly of epithelioid cells was established from the caudal fin of marbled eels ( Anguilla marmorata ) and designated as marbled eel caudal fin ( MECF )‐1. The cells multiplied well in Leibovitz's L‐15 medium containing 2% to 15% foetal bovine serum at temperatures of 20°C to 35°C and were subcultured for >90 passages during a 5‐year period from 2012 to 2017. Transcripts of ictacalcin, keratin 13 , cd146 , nestin , ncam1 and myod1 were demonstrated in the cells using reverse transcription polymerase chain reaction. The results indicated that MECF ‐1 was composed of epidermal and mesenchyme stem and progenitor cells including myoblasts. MECF ‐1 was susceptible to Japanese eel herpesvirus HVA 980811, marbled eel polyoma‐like virus ( MEP yV), aquabirnavirus MEIPNV 1310 and aquareovirus CSV . By contrast, MECF ‐1 was noted refractory to megalocytiviruses RSIV ‐Ku and GSIV ‐K1 infection. Moreover, the cells were resistant to betanodavirus infection. In conclusion, MECF ‐1 derived from marbled eel is suitable for studies on anguillid viruses and interaction with host cells.