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Establishment of a cell line from the kidney of black carp and its susceptibility to spring viremia of carp virus
Author(s) -
Xue T,
Wang Y Z,
Pan Q H,
Wang Q,
Yuan J F,
Chen T S
Publication year - 2018
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12736
Subject(s) - biology , viremia , carp , grass carp , common carp , cell culture , virology , microbiology and biotechnology , virus , minnow , transfection , crucian carp , pathogen , genetics , fishery , fish <actinopterygii> , cyprinus
In this study, we established and characterized a cell line derived from the kidney of black carp ( Mylopharyngodon piceus ), which is an important freshwater aquaculture species. The cell line was designated as MPK and subcultured for more than 70 passages in DMEM medium containing 10% fetal bovine serum ( FBS ) at 28°C. MPK had a modal diploid chromosome number of 48. Moreover, a transient MPK transfection efficiency was up to 18% using a green fluorescent protein plasmid by a modified electroporation. In addition, the MPK cells showed susceptibility to spring viremia of carp virus ( SVCV ), as demonstrated by the presence of severe cytopathic effects ( CPE s) and increased viral RNA . Unexpectedly, the MPK cells expressed pluripotency‐associated genes such as nanog , oct 4 and vasa , indicating that these are possibly adult stem cells. Taken together, we have established a stable cell line from kidney that may potentially be utilized as an in vitro platform for genetic modifications and host–pathogen analysis in black carp.