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Serum adenosine deaminase and xanthine oxidase activities in silver catfish naturally infected with Ichthyophthirius multifiliis : The influence of these enzymes on inflammatory and oxidative status
Author(s) -
Baldissera M D,
Souza C F,
Doleski P H,
Monteiro S G,
Silva A S,
Baldisserotto B
Publication year - 2018
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12709
Subject(s) - ichthyophthirius multifiliis , uric acid , catfish , xanthine oxidase , adenosine deaminase , downregulation and upregulation , biology , reactive oxygen species , nitric oxide , oxidative stress , microbiology and biotechnology , respiratory burst , immunology , adenosine , biochemistry , enzyme , endocrinology , fishery , fish <actinopterygii> , gene
Abstract Adenosine deaminase ( ADA ) activity, through adenosine (Ado) levels, as well as xanthine oxidase ( XO ) activity through uric acid levels exerts an essential role on immune and inflammatory responses during infectious diseases. Thus, the aim of this study was to evaluate the involvement of seric ADA and XO activities in the inflammatory and oxidative status of silver catfish naturally infected with Ichthyophthirius multifiliis . Seric ADA activity decreased, while Ado levels increased in infected animals compared to uninfected animals. Moreover, the seric XO activity increased in infected animals compared to uninfected animals, alongside the seric levels of uric acid, metabolites of nitric oxide ( NO x) and reactive oxygen species ( ROS ). Based on this evidence, the downregulation of seric ADA activity exerts an anti‐inflammatory profile, contributing to restricting the inflammatory process. The most important finding is that upregulation of seric XO activity leads to an excessive formation of uric acid, which contributes to oxidative and inflammatory processes. Moreover, uric acid induces the release of pro‐inflammatory and pro‐oxidative mediators, such NO x and ROS , which contribute directly to disease pathogenesis. In summary, the upregulation of XO activity may be considered a pathway involved in NO x and ROS production in silver catfish infected with I. multifiliis .