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Rainbow trout ( Oncorhynchus mykiss ) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis
Author(s) -
Gersdorff Jørgensen L,
Kania P W,
Rasmussen K J,
Mattsson A H,
Schmidt J,
AlJubury A,
Sander A,
Salanti A,
Buchmann K
Publication year - 2017
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12653
Subject(s) - ichthyophthirius multifiliis , biology , rainbow trout , microbiology and biotechnology , recombinant dna , parasite hosting , immune system , virology , immunity , antibody , antigen , immunology , fishery , fish <actinopterygii> , biochemistry , world wide web , computer science , gene
The protective effect in rainbow trout ( Oncorhynchus mykiss ) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n‐terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I‐antigen in future vaccine trials.