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Clonal structure in Ichthyobacterium seriolicida , the causative agent of bacterial haemolytic jaundice in yellowtail, Seriola quinqueradiata , inferred from molecular epidemiological analysis
Author(s) -
Matsuyama T,
Fukuda Y,
Sakai T,
Tanimoto N,
Nakanishi M,
Nakamura Y,
Takano T,
Nakayasu C
Publication year - 2017
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12586
Subject(s) - multiple loci vntr analysis , biology , amplified fragment length polymorphism , seriola quinqueradiata , multilocus sequence typing , genetics , housekeeping gene , variable number tandem repeat , tandem repeat , gene , genotype , genome , fishery , genetic diversity , population , gene expression , demography , sociology , fish <actinopterygii>
Bacterial haemolytic jaundice caused by Ichthyobacterium seriolicida has been responsible for mortality in farmed yellowtail, Seriola quinqueradiata , in western Japan since the 1980s. In this study, polymorphic analysis of I. seriolicida was performed using three molecular methods: amplified fragment length polymorphism ( AFLP ) analysis, multilocus sequence typing ( MLST ) and multiple‐locus variable‐number tandem repeat analysis ( MLVA ). Twenty‐eight isolates were analysed using AFLP , while 31 isolates were examined by MLST and MLVA . No polymorphisms were identified by AFLP analysis using Eco RI and Mse I, or by MLST of internal fragments of eight housekeeping genes. However, MLVA revealed variation in repeat numbers of three elements, allowing separation of the isolates into 16 sequence types. The unweighted pair group method using arithmetic averages cluster analysis of the MLVA data identified four major clusters, and all isolates belonged to clonal complexes. It is likely that I. seriolicida populations share a common ancestor, which may be a recently introduced strain.