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Aluminium chloride‐induced toxicity in zebrafish larvae
Author(s) -
Monaco A,
Grimaldi M C,
Ferrandino I
Publication year - 2017
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12544
Subject(s) - toxicity , biology , glial fibrillary acidic protein , larva , chloride , cadmium chloride , zebrafish , toxicology , andrology , anatomy , biochemistry , cadmium , chemistry , medicine , botany , immunology , immunohistochemistry , organic chemistry , gene
Embryos at shield stage and larvae at protruding mouth stage were exposed to different concentrations of aluminium chloride (AlCl 3 ) for 72 h with the purpose to analyse their phenotype and lethality. After 24, 48 and 72 h of treatment, higher toxicity of the metal was observed on larvae with minimal lethal concentration of 0.25, 0.20 and 0.08 m m , respectively, while for embryos the corresponding values were 40, 25 and 16 m m . We observed pericardial oedema and alteration of heart rate in 50% of larvae after 48 h of exposure to 100 μ m . In larvae exposed to the same concentration, there was also a neurological injury at the level of glial cells, with the number of glial fibrillary acidic protein‐positive cells being significantly reduced. This study confirms the toxic nature of this metal and shows that aluminium could also interestingly represent a cardiotoxin in addition to its neurotoxic ability.