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Cloning and expression analysis of innate immune genes from red sea bream to assess different susceptibility to megalocytivirus infection
Author(s) -
Jin J W,
Kim Y C,
Hong S,
Kim M S,
Jeong J B,
Jeong H D
Publication year - 2017
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12537
Subject(s) - biology , pagrus major , innate immune system , gene , immune system , cytopathic effect , cloning (programming) , gene expression , virology , microbiology and biotechnology , immunology , virus , genetics , fishery , fish <actinopterygii> , computer science , programming language
As suggested by the Office International des Epizooties ( OIE ), fishes belonging to the genus Oplegnathus are more sensitive to megalocytivirus infection than other fish species including red sea bream ( Pagrus major ). To assess the roles of the innate immune response to these different susceptibilities, we cloned the genes encoding inflammatory factors including IL ‐8 and COX ‐2, and the antiviral factor like Mx from red sea bream for the first time and performed phylogenetic and structural analysis. Analysed expression levels of IL ‐1β, IL ‐8 and COX ‐2 and the antiviral factor like Mx genes performed with in vivo challenge experiment showed no difference in inflammatory gene expression or respiratory burst activity between red sea bream and rock bream ( Oplegnathus fasciatus ). However, the Mx gene expression levels in red sea bream were markedly higher than those in rock bream, suggesting the importance of type I interferon ( IFN )‐induced proteins, particularly Mx, during megalocytivirus infection, rather than inflammation‐related genes. The in vitro challenge experiments using embryonic primary cultures derived from both fish species showed no difference in cytopathic effects ( CPE ), viral replication profiles, and inflammatory and Mx gene expression pattern between the two fish species.