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Development and validation of glycoprotein‐based native‐subunit vaccine for fish against A eromonas hydrophila
Author(s) -
Çiftci A,
Onuk E E,
Çiftci G,
Fındık A,
Söğüt M Ü,
Didinen B I,
Aksoy A,
Üstünakın K,
Gülhan T,
Balta F,
Altun S
Publication year - 2016
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12499
Subject(s) - aeromonas hydrophila , glycoprotein , biology , microbiology and biotechnology , ginseng , lectin , glycan , fish <actinopterygii> , fishery , medicine , alternative medicine , pathology
Aeromonas hydrophila is known to be causative agent of an infection named as Bacterial haemorrhagic septicaemia or red pest in freshwater fish. The aim of this study was to develop and validate the glycoprotein‐based fish vaccine against Aeromonas hydrophila . For this aim, after identification and characterization of A. hydrophila isolates from fish farms, one A. hydrophila isolate was selected as vaccine strain. Antigenic glycoproteins of this vaccine strain were determined by Western blotting and glycan detection kit. The connection types of these glycoproteins were examined by glycoprotein differentiation kit. Two glycoproteins, molecular weights of 19 and 38 kD a, with SNA connection type were selected for use in vaccination trials. After their purification by SNA ‐specific lectin and size‐exclusion chromatography, protection studies with purified proteins were performed. For challenge trials, four experimental fish groups were designated: Group I (with montanide), Group II (with montanide and ginseng), Group III [with Al( OH ) 3 ] and Group IV [with Al( OH ) 3 and ginseng]. The survival ratings of fish were determined, and protection was calculated as 21.56%, 29.41%, 69.83% and 78.88% in groups I, II , III and IV , respectively. In conclusion, A. hydrophila glycoproteins with Al( OH )3 and ginseng could be used as a safe and effective vaccine for fish.