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First detection of koi herpesvirus from koi, Cyprinus carpio L. experiencing mass mortalities in Iran: clinical, histopathological and molecular study
Author(s) -
RahmatiHolasoo H,
Zargar A,
Ahmadivand S,
Shokrpoor S,
Ezhari S,
Ebrahimzadeh Mousavi H A
Publication year - 2016
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12448
Subject(s) - cyprinus , biology , gill , common carp , spleen , carp , genotype , necrosis , mucus , pathology , histopathological examination , virology , gene , fish <actinopterygii> , immunology , genetics , fishery , medicine , ecology
Abstract Koi herpesvirus ( KHV ) is the aetiological agent of an emerging disease ( KHVD ) associated with mass mortalities in koi and common carp and reported from at least 30 countries. We report the first detection of KHV from koi in Iran using clinical, histopathological and molecular studies. KHV ‐infected fish showed reduced swimming activity, sunken eyes and increased mucus production on skin and fins. On post‐mortem examination, gill necrosis was observed in the majority of fish. Histopathologically, the gill showed diffuse necrosis of the branchial epithelial cells. Margination of chromatin was detected in gills, kidney, heart, spleen, intestine and brain. In addition, sequence analyses of the TK gene, ORF 136 and marker I and II , demonstrates that Iranian KHV isolates were identical and classified as variant A1 of TUSMT 1 (J strain) and displayed the I ++ II + allele of this Asian genotype.