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Developing immunological methods for detecting Macrobrachium rosenbergii nodavirus and extra small virus using a recombinant protein preparation
Author(s) -
Wang CS,
Chang CY,
Wen CM
Publication year - 2016
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12404
Subject(s) - biology , polyclonal antibodies , virology , microbiology and biotechnology , recombinant dna , western blot , virus , blot , fusion protein , antibody , dot blot , gene , immunology , biochemistry
Macrobrachium rosenbergii nodavirus ( Mr NV ) and extra small virus ( XSV ) have been identified as the causative agents for white tail disease ( WTD ) of M. rosenbergii . In this study, the gene sequences encoding Mr NV and XSV capsid proteins were separately ligated into the pGEX ‐4T‐3 expression vector and transformed into Escherichia coli . After induction, glutathione‐S‐transferase ( GST )‐tagged Mr NV and XSV fusion proteins were obtained with molecular masses of 68 and 43 kD a, respectively. Specific polyclonal antibodies for Mr NV and XSV against viral recombinant proteins and infected prawn tissues were verified using Western blotting. According to immunodot blot assay results, the detection sensitivities of antibodies were approximately 5 ng μL −1 for both recombinant proteins GST ‐ Mr NV and GST ‐ XSV . In additional, Mr NV and XSV were detected at dilution levels of 1:2560 and 1:640 in the infected prawn tissues, respectively. No cross‐reactions with white spot syndrome virus or grouper nervous necrosis virus were observed using immunodot blot assays. Mr NV and XSV in infected muscle tissues were detected using immunohistochemistry. Although the detection limit of the immunodot blot assay was lower than that of nested reverse transcription polymerase chain reaction, these polyclonal antibodies can be useful for confirming Mr NV and XSV infections in field tests.