Premium
Characterization of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in southern Thailand
Author(s) -
Kongrueng J,
Yingkajorn M,
Bunpa S,
Sermwittayawong N,
Singkhamanan K,
Vuddhakul V
Publication year - 2015
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12308
Subject(s) - vibrio parahaemolyticus , biology , microbiology and biotechnology , serotype , virulence , plasmid , outbreak , toxin , virology , vibrio infections , hemolysin , gene , bacteria , genetics
Vibrio parahaemolyticus was isolated from shrimp of five farms located in the Pattani and Songkhla provinces of southern Thailand. Using a PCR method targeted to the unique DNA sequences derived from the plasmid ( AP 2 primers) and the toxin gene ( AP 3 primers) of V. parahaemolyticus that caused acute hepatopancreatic necrosis disease ( AHPND ), a total of 33 of 108 isolates were positive. In contrast, all 63 and 66 isolates of clinical and environmental V. parahaemolyticus, respectively, obtained previously from 2008 to 2014 from the same area were negative. This implied that these strains were likely to be the cause of the outbreak of AHPND in this area. Intestinal samples proved to be a better source for the isolation of V. parahaemolyticus AHPND than the hepatopancreas. All isolates were investigated for haemolytic activity, virulence genes, serotypes, genotypes and antibiotic susceptibility. All the AHPND isolates had a unique O antigen, but small variations of the K antigens were detected from different farms. In addition, the DNA profiles of V. parahaemolyticus AHPND isolates were similar, but distinct from those clinical and environmental isolates. It is postulated that the causative agent of AHPND might have originated from one clone and then slightly different serotypes subsequently developed.