Premium
Isolation and screening of probiotic candidates from marron, C herax cainii ( A ustin, 2002) gastrointestinal tract ( GIT ) and commercial probiotic products for the use in marron culture
Author(s) -
Ambas I,
Buller N,
Fotedar R
Publication year - 2015
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12257
Subject(s) - probiotic , microbiology and biotechnology , biology , bacteria , penicillin , protease , bacillus subtilis , antibiotics , enzyme , biochemistry , genetics
Six strains of bacteria including B acillus mycoides (A10) and Shewanella species (A12) isolated from healthy marron intestine, B acillus species ( PM 1), B acillus subtilis ( PM 3), B acillus sp. ( PM 4) and B acillus sp. ( AQ ) from commercial probiotic products were investigated for probiotic potential in marron culture. Antibiotic susceptibility tests indicated PM 3 and PM 4 were susceptible to all nine antibiotics evaluated. A10, A12 and AQ were resistant to class penicillins, whereas PM 1 was resistant to class penicillin and macrolides. All strains were non‐pathogenic for marron. Strong inhibition against V ibrio mimicus and V ibrio cholerae non‐01 was exhibited by PM 4 and PM 3. A10 inhibited V . mimicus at 72 h of growth, but not V . cholerae non‐01, whereas A12 inhibited V . cholerae non‐01 but not V . mimicus , and AQ showed no inhibition activity. A wide range of enzymes were produced by A10 and AQ using the API ZYM test. Protease enzymes were produced by PM 3, PM 4, AQ and PM 1. In order of effectiveness, the following bacteria have probiotic potential : B . subtilis ( PM 3), Bacillus sp. ( PM 4) and B . mycoides (A10). Further study is required to determine the bacterium or any combination that gives a multibeneficial effect on marron.