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Phenotypical and genotypical characterization of S hewanella putrefaciens strains isolated from diseased freshwater fish
Author(s) -
Pękala A,
Kozińska A,
Paździor E,
Głowacka H
Publication year - 2015
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12231
Subject(s) - biology , rainbow trout , shewanella putrefaciens , cyprinus , common carp , carp , gill , freshwater fish , outbreak , aeromonas , microbiology and biotechnology , fish farming , veterinary medicine , zoology , bacteria , fish <actinopterygii> , fishery , aquaculture , virology , medicine , genetics
Between 2007 and 2012, a variety of disease outbreaks most often characterized by skin disorders were observed among different species of freshwater fish in Poland. In most cases, the clinical signs included focally necrotized gills, necrotic skin lesions or ulcers. Internally, haemorrhages, oedematous kidney and abnormal spleen enlargement were generally noted. The disorders were accompanied by increased mortality. Most of the problems concerned cultured common carp C yprinus carpio L. and rainbow trout O ncorhynchus mykiss (Walbaum). Fish have been examined from a number of these farms, and additionally, the wild and ornamental fish with similar clinical signs of diseases were also tested. Bacteria were isolated consistently from lesions and internal organs. They had characteristic orange‐pigmented colonies which grew in pure culture or constituted 55–95% of total bacterial flora. One hundred and eighteen isolates were collected and biochemically identified as S hewanella putrefaciens group, and this was confirmed by sequencing. Challenge tests confirmed the pathogenicity of these bacteria. This is the first report characterizing and describing S . putrefaciens as a pathogen of different species of freshwater fish in Europe.