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Immunohistochemical diagnosis of tenacibaculosis in paraffin‐embedded tissues of S enegalese sole S olea senegalensis K aup, 1858
Author(s) -
Faílde L D,
Bermúdez R,
Losada A P,
Riaza A,
Santos Y,
Quiroga M I
Publication year - 2014
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12199
Subject(s) - biology , immunohistochemistry , immunostaining , inoculation , antigen , outbreak , pathology , spleen , lesion , fish <actinopterygii> , pathogenesis , microbiology and biotechnology , immunology , virology , medicine , fishery
A sensitive and specific immunohistochemical technique was developed to improve the diagnosis of tenacibaculosis and to better understand its pathogenesis. Senegalese sole S olea senegalensis K aup, 1858 were inoculated subcutaneously with a bacterial suspension of T enacibaculum maritimum, and samples were taken at different hours post‐inoculation. Sections from different organs were used as positive controls. In addition, a total of 128 field samples from different organs collected from tenacibaculosis outbreaks were used. Tenacibaculum maritimum antigens were detected in several organs of experimentally infected S enegalese sole and in at least one of the tissues from fish suffering from natural tenacibaculosis previously confirmed by culture and PCR ‐based methods. In fish collected during outbreaks, a strong positive reaction was detected in ulcerative skin areas. Moreover, bacterial antigen was identified inside scale pockets and in sites of the skin with mild lesion. In kidney and spleen, evident immunostaining of bacterial antigen was detected in both naturally and experimentally infected fish. Besides, the presence of T . maritimum in the intestinal tract without associated histological changes suggests that this organ may act as a reservoir for T . maritimum . The results of this study confirm the usefulness of IHC for the diagnosis of tenacibaculosis in paraffin‐embedded tissues.