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Comparison of pulsed‐field gel electrophoresis and enterobacterial repetitive intergenic consensus PCR and biochemical tests to characterize L actococcus garvieae
Author(s) -
Ture M,
Altinok I,
Capkin E
Publication year - 2015
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12192
Subject(s) - intergenic region , pulsed field gel electrophoresis , biology , typing , gel electrophoresis , polymerase chain reaction , strain (injury) , genomic dna , microbiology and biotechnology , restriction enzyme , dna , genetics , genotype , gene , genome , anatomy
Biochemical test, pulsed‐field gel electrophoresis ( PFGE ) and enterobacterial repetitive intergenic consensus sequence PCR ( ERIC ‐ PCR ) were used to compare 42 strains of L actococcus garvieae isolated from different regions of T urkey, I taly, F rance and S pain. Twenty biotypes of L . garvieae were formed based on 54 biochemical tests. ERIC ‐ PCR of genomic DNA from different L . garvieae strains resulted in amplification of multiple fragments of DNA in sizes ranging between 200 and 5000 bp with various band intensities. After cutting DNA with Apa I restriction enzyme and running on the PFGE , 11–22 resolvable bands ranging from 2 to 194 kb were observed. T urkish isolates were grouped into two clusters, and only A 58 ( I taly) strain was connected with T urkish isolates. Similarities between T urkish, S panish, I talian and F rench isolates were <50% except 216‐6 Rize strain. In T urkey, first lactococcosis occurred in M ugla, and then, it has been spread all over the country. Based on ERIC ‐ PCR , S panish and Italian strains of L . garvieae were related to M ugla strains. Therefore, after comparing PFGE profiles, ERIC ‐ PCR profiles and phenotypic characteristics of 42 strains of L . garvieae , there were no relationships found between these three typing methods. PFGE method was more discriminative than the other methods.