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Multilocus variable number tandem repeat analysis of E dwardsiella piscicida isolates pathogenic to fish
Author(s) -
Abayneh T,
Colquhoun D J,
Austin D,
Sørum H
Publication year - 2014
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12190
Subject(s) - multiple loci vntr analysis , variable number tandem repeat , biology , tandem repeat , genotyping , minisatellite , genetics , typing , multilocus sequence typing , microsatellite , genotype , allele , genome , gene
This study describes a novel multilocus variable number tandem repeat analysis ( MLVA ) based on six variable number of tandem repeat ( VNTR ) loci for genotyping of 37 E dwardsiella piscicida (previously E dwardsiella tarda ) isolates from multiple sources. The number of alleles identified for each of the six VNTR loci ranged from 3 to 5 with VNTR loci 1 ( DI = 0.632) and 3 ( DI = 0.644), displaying the highest degrees of polymorphism. MLVA typing of the 37 E . piscicida isolates resulted in the identification of five major clusters consistent with their geographical origins, and were designated as MLVA types I , II , III , IV and V . Types III and V were resolved further into subtypes largely consistent with outbreak source. An MLVA profile comprising a string of integers representing the number of tandem repeats for each allele provided a unique identification for each MLVA type and/or strain. The MLVA protocol described in the current study is robust, relatively simple, has a higher power of resolution than multilocus sequence analysis ( MLSA ) and is capable of discriminating closely related isolates.