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Tissue distribution of hepatopancreatic parvo‐like virus of shrimp in freshwater rice‐field crab, P aratelphusa hydrodomous ( H erbst)
Author(s) -
Madan N,
Rajkumar T,
Sundar Raj N,
Farook M A,
Nambi K S N,
Abdul Majeed S,
Sahul Hameed A S
Publication year - 2014
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12183
Subject(s) - biology , shrimp , virus , western blot , decapoda , virology , shellfish , freshwater crab , capsid , microbiology and biotechnology , fishery , aquatic animal , crustacean , gene , fish <actinopterygii> , genetics
An attempt was made to determine the replication efficiency of hepatopancreatic parvo‐like virus ( HPV ) of shrimp in different organs of freshwater rice‐field crab P aratelphusa hydrodomous ( H erbst) using bioassay, PCR , RT ‐ PCR , ELISA , Western blot and q‐ PCR analyses. Another attempt was made to use this crab as an alternative to penaeid shrimp for the large‐scale production of HPV . This crab was found to be highly susceptible to HPV by intramuscular injection. The systemic HPV infection was confirmed by PCR and W estern blot analyses in freshwater crab. The expression of capsid protein gene in different organs of infected crab was revealed by RT ‐ PCR analysis. Indirect ELISA was used to quantify the capsid protein in different organs of the crab. The copy number of HPV in different organs of the infected crab was quantified by q‐ PCR . The results revealed a steady decrease in C T values in different organs of the infected crab during the course of infection. The viral inoculum that was prepared from different organs of the infected crab caused significant mortality in post‐larvae of tiger prawn, P enaeus monodon ( F abricius). The results revealed that this rice‐field crab could be used as an alternative host for HPV replication and also for large‐scale production of HPV .