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Development of a selective and differential medium for capsulated L actococcus garvieae
Author(s) -
Chang CI,
Lee CF,
Tsai JM,
Wu CC,
Chen LH,
Chen SC,
Lin KJ
Publication year - 2014
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12162
Subject(s) - biology , microbiology and biotechnology , agar , enterococcus , enterococcus faecium , agar plate , enterococcus faecalis , isolation (microbiology) , vibrio , vibrio anguillarum , bacteria , staphylococcus aureus , antibiotics , genetics
A selective and differential medium termed ‘ LG agar’ was developed for the isolation and presumptive identification of L actococcus garvieae that results in black colonies with red halos. In this study, all 14 strains of L . garvieae and only 9 of the 148 strains representing 38 other species were able to grow on the LG agar. The nine viable strains on LG agar plates (including E nterococcus faecalis , E nterococcus faecium , L actococcus lactis , V ibrio fluvialis , V ibrio furnissii , V ibrio mimicus and V ibrio salmonicida ) were further differentiated from L . garvieae by various colours or colony features. Colonies isolated from the mixing culture and the infected giant sea perch using LG agar plates were all positively identified as L . garvieae by conventional tests and 16 S r DNA sequencing. Furthermore, LG agar discriminated capsulated strains of L . garvieae , which were believed to be correlated with pathogens of fish and shellfish, from non‐capsulated ones by colony appearances. The specificity and differentiating ability of LG agar suggest that this medium displays considerable potential for primary isolation and presumptive identification of L . garvieae from pathological and environmental samples.