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Relative prevalence of Mycobacterium marinum in fish collected from aquaria and natural freshwaters in central Europe
Author(s) -
Slany M,
Makovcova J,
Jezek P,
Bodnarova M,
Pavlik I
Publication year - 2014
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12135
Subject(s) - mycobacterium marinum , biology , gill , fish <actinopterygii> , veterinary medicine , fish farming , aquatic animal , fishery , mycobacterium , aquaculture , bacteria , medicine , genetics
A survey was carried out on occurrence of Mycobacterium marinum in fish kept in aquaria and those living in their natural environment. Species‐specific qPCR targeting the erp and IS 2404 genes together with the conventional culture method were used. The analysis of 72 ornamental fish ( n = 216 samples: gills, muscle and intestine) collected from aquaria revealed the presence of M. marinum in 30 individuals (41.7%) of whom 17 (23.6%) were later culture positive. Culture‐independent detection revealed the presence of M. marinum in 16 of 83 environmental samples (19.3%) collected in aquaria. The presence of viable M. marinum cells was later confirmed in 5 samples (6.0%). No qPCR or culture positivity was observed when 123 groundwater fish and their corresponding environmental samples ( n = 142) were analysed.