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A new cultivation‐independent tool for fast and reliable detection of M ycobacterium marinum
Author(s) -
Slany M
Publication year - 2014
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12113
Subject(s) - mycobacterium marinum , biology , enumeration , fish <actinopterygii> , microbiology and biotechnology , mycobacterium , bacteria , fishery , genetics , combinatorics , mathematics
The M ycobacterium marinum group ( MMG ) is a class of mycobacteria that includes M . marinum , the cause of chronic systemic infections in fish. This species occasionally causes granulomatous skin lesions in humans. Other members of MMG are mycolactone‐producing mycobacteria ( MPM ; M . ulcerans , M . shottsii and M . pseudoshottsii ). The cultivation‐independent approach presented in this study brings a fast and reliable alternative to classically used cultivation methods. The developed triplex erp / IS 2404 q PCR assay is based on a primary species‐specific erp detection, which allows enumeration of MMG in analysed samples, and secondary IS 2404 detection is suitable for the differentiation of M . marinum from MPM . The detection of M . marinum in clinical specimens and in artificially contaminated tissue samples has proven its applicability for diagnostic purposes.