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Visceral granulomas in farmed large yellow croaker, Larimichthys crocea (Richardson), caused by a bacterial pathogen, Pseudomonas plecoglossicida
Author(s) -
Zhang J T,
Zhou S M,
An S W,
Chen L,
Wang G L
Publication year - 2014
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12075
Subject(s) - biology , pathogen , microbiology and biotechnology , 16s ribosomal rna , virulence , strain (injury) , enzootic , ribosomal rna , phylogenetic tree , gene , sequence analysis , bacteria , virology , genetics , anatomy , virus
An enzootic disease characterized by granulomas in internal organs occurred in cage‐farmed large yellow croaker, L arimichthys crocea ( R ichardson), in A pril and N ovember 2010, in N ingbo, Z hejiang P rovince. One bacterial strain, named XSDHY‐P, was isolated from the diseased fish and identified by biochemical characterization, fatty acid methyl ester ( FAME ) analysis and multilocus sequence analysis ( MLSA ). According to the results obtained from the biochemical tests, FAME analysis and phylogenetic analysis derived from 16S ribosomal RNA , gyrB , oprF , oprI , oprL and rpoD gene sequencing, the bacterial isolate, XSDHY‐P , was identified as P seudomonas plecoglossicida . Moreover, lethal dose, 50% trials were carried out to demonstrate the virulence of XSDHY‐P in large yellow croaker when administered at 2.13 × 10 5 colony‐forming units per fish. Visceral granulomas were found in the experimentally infected fish as well as in the naturally infected fish, indicating that P . plecoglossicida is another bacterial pathogen that causes granulomatosis in L . crocea .