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Antibody screening identifies 78 putative host proteins involved in C yprinid herpesvirus 3 infection or propagation in common carp, C yprinus carpio L
Author(s) -
Gotesman M,
Soliman H,
ElMatbouli M
Publication year - 2013
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12073
Subject(s) - common carp , biology , cyprinus , virology , antibody , bovine herpesvirus 1 , monoclonal antibody , carp , virus , microbiology and biotechnology , herpesviridae , viral disease , immunology , fish <actinopterygii> , fishery
Abstract Cyprinid herpesvirus 3 ( C y HV ‐3) is the aetiological agent of a serious and notifiable disease afflicting common and koi carp, Cyprinus carpio L., termed koi herpesvirus disease ( KHVD ). Significant progress has been achieved in the last 15 years, since the initial reports surfaced from G ermany, USA and Israel of the C y HV ‐3 virus, in terms of pathology and detection. However, relatively few studies have been carried out in understanding viral replication and propagation. Antibody‐based affinity has been used for detection of C y HV ‐3 in enzyme‐linked immunosorbent assay and PCR ‐based techniques, and immunohistological assays have been used to describe a C y HV ‐3 membrane protein, termed ORF 81. In this study, monoclonal antibodies linked to N ‐hydroxysuccinimide ( NHS )‐activated spin columns were used to purify C y HV ‐3 and host proteins from tissue samples originating in either C y HV ‐3 symptomatic or asymptomatic fish. The samples were next analysed either by polyacrylamide gel electrophoresis ( PAGE ) and subsequently by electrospray ionization coupled to mass spectrometry ( ESI ‐ MS ) or by ESI ‐ MS analysis directly after purification. A total of 78 host proteins and five C y HV ‐3 proteins were identified in the two analyses. These data can be used to develop novel control methods for Cy HV ‐3, based on pathways or proteins identified in this study.