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Full‐length cloning and phylogenetic analyses of translationally controlled tumour protein and ferritin genes from the Indian white prawn, F enneropenaeus indicus ( H . M ilne E dwards)
Author(s) -
Nayak S,
Ramaiah N,
Meena R M,
Sreepada R A
Publication year - 2014
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12040
Subject(s) - biology , orfs , gene , vibrio harveyi , open reading frame , genetics , phylogenetic tree , cloning (programming) , innate immune system , white spot syndrome , prawn , ferritin , microbiology and biotechnology , immune system , vibrio , peptide sequence , bacteria , biochemistry , fishery , computer science , programming language
Abstract Elucidation, through molecular analyses, of bacterial afflictions in commercially important aquaculture‐reared shrimps is pivotal for the prevention and/or control of disease outbreaks. In this study, we examined the phylogenetic relatedness and compared the possible immune‐related functional roles of both translationally controlled tumour protein ( TCTP ) and ferritin genes with previous studies. Both TCTP and ferritin genes were substantially upregulated in the Indian white prawn, F enneropenaeus indicus (H. Milne Edwards), post‐larvae following bath challenge with the virulent strain of bacteria, V ibrio harveyi D 3. Full‐length cloning of these genes by rapid amplification of complementary DNA ends ‐polymerase chain reaction (RACE‐PCR) yielded 727‐base pair (bp)‐long TCTP and 1212‐bp‐long ferritin gene sequences. Their open reading frames ( ORF s) were 507 and 510 bp, respectively. The TCTP ‐ ORF coded for 168 amino acids with three substitutions at positions 37, 141, 155, and the ferritin ORF coded for 170 amino acids with no species‐specific substitutions. Phylogenetic analysis suggested the closest relatedness of both TCTP and ferritin from F . indicus to Chinese white prawn, Fenneropenaeus chinensis (Osbeck). In addition to reporting the full‐length sequences of these immune‐relevant genes, this study highlighted their conserved natures, which perhaps make them important defence‐related proteins in the innate immune system of F . indicus .