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Cloning, expression of V ibrio alginolyticus outer membrane protein‐ O mp U gene and its potential application as vaccine in c rimson snapper, L utjanus erythropterus B loch
Author(s) -
Cai S H,
Lu Y S,
Wu Z H,
Jian J C
Publication year - 2013
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12036
Subject(s) - vibrio alginolyticus , recombinant dna , biology , immunogenicity , polyclonal antibodies , virulence , microbiology and biotechnology , cloning (programming) , bacterial outer membrane , antibody , escherichia coli , virology , gene , vibrio , bacteria , biochemistry , immunology , genetics , computer science , programming language
The outer membrane proteins of the marine aquatic animal pathogen, V ibrio alginolyticus , play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In this study, the gene encoding an outer membrane protein‐ O mp U was cloned and expressed in E scherichia coli . Polyclonal antibodies were raised in rabbits against the purified recombinant O mp U , and the reaction of the antibody was confirmed by Western blotting using the isolated O mp U and the recombinant O mp U of V . alginolyticus . To analyze the immunogenicity of the recombinant O mp U , c rimson snapper, L utjanus erythropterus B loch, were immunized by intraperitoneal injection, and antibody response was assessed by the enzyme‐linked immunosorbent assay ( ELISA ). The results demonstrated that the recombinant O mp U produced an observable antibody response in all sera of the vaccinated fish. The vaccinated fish were challenged by virulent V . alginolyticus and observed to have high resistance to infection. These results indicate that the recombinant O mp U is an effective vaccine candidate against V . alginolyticus in L . erythropterus .