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Luminal uptake of V ibrio ( L istonella ) anguillarum by shed enterocytes – a novel early defence strategy in larval fish
Author(s) -
Rekecki A,
Ringø E,
Olsen R,
Myklebust R,
Dierckens K,
Bergh Ø,
Laureau S,
Cornelissen M,
Ducatelle R,
Decostere A,
Bossier P,
den Broeck W
Publication year - 2013
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12009
Subject(s) - biology , vibrio anguillarum , enterocyte , microbiology and biotechnology , sea bass , dicentrarchus , immunogold labelling , brush border , virulence , pathogen , bacteria , vesicle , anatomy , biochemistry , ultrastructure , fishery , membrane , small intestine , fish <actinopterygii> , genetics , vibrio , gene
Abstract As adhesion and translocation through fish gut enterocytes of the pathogen V ibrio ( L istonella) anguillarum are not well investigated, the effective cause of disease and mortality outbreaks in larval sea bass, D icentrarchus labrax , suffering from vibriosis is unknown. We detected V .  anguillarum within the gut of experimentally infected gnotobiotic sea bass larvae using transmission electron microscopy and immunogold labelling. Intact bacteria were observed in close contact with the apical brush border in the gut lumen. Enterocytes contained lysosomes positive for protein A ‐gold particles suggesting intracellular elimination of bacterial fragments. Shed intestinal cells were regularly visualized in the gut lumen in late stages of exposure. Some of the luminal cells showed invagination and putative engulfment of bacterial structures by pseudopod‐like formations. The engulfed structures were positive for protein A ‐colloidal gold indicating that these structures were V . anguillarum . Immunogold positive thread‐like structures secreted by V . anguillarum suggested the presence of outer membrane vesicles ( MV s) hypothesizing that MV s are potent transporters of active virulence factors to sea bass gut cells suggestive for a substantial role in biofilm formation and pathogenesis. We put forward the hypothesis that MV s are important in the pathogenesis of V .  anguillarum in sea bass larvae.

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