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Inhibition of hirame rhabdovirus growth by RNA aptamers
Author(s) -
Hwang S D,
Midorikawa N,
Punnarak P,
Kikuchi Y,
Kondo H,
Hirono I,
Aoki T
Publication year - 2012
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/jfd.12000
Subject(s) - aptamer , systematic evolution of ligands by exponential enrichment , rna , biology , selex aptamer technique , nucleic acid , virology , virus , in vitro , rna interference , recombinant dna , microbiology and biotechnology , gene , biochemistry
RNA aptamers are artificial nucleic acids that specifically bind to a wide variety of targets. They are an effective tool for pharmaceutical research and development of antiviral agents. Here, we describe four Hirame rhabdovirus ( HIRRV )‐ RNA aptamers ( H 1, H 2, H 3 and H 4) that we obtained from an in vitro process called the systematic evolution of ligands by exponential enrichment ( SELEX ). The HIRRV ‐ RNA aptamers specifically bind to HIRRV . Hirame natural embryo ( HINAE ) cells treated with virus and the RNA aptamer showed a decrease in appearance of cytopathic effect when compared with control (treated only with virus). Rhodovulum sulfidophilum was transformed with genes for the RNA aptamers, and the aptamers were detected in the culture medium, indicating that they were secreted from the cells. Thus, the recombinant R . sulfidophilum might be a powerful tool for the prevention of HIRRV in aquaculture.