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Antiproliferative effects of Turkish pomegranate ( Punica granatum L.) extracts on MCF‐7 human breast cancer cell lines with focus on antioxidant potential and bioactive compounds analyzed by LC‐MS/MS
Author(s) -
Eroglu Ozkan Esra,
Seyhan Mehmet Fatih,
Kurt Sirin Ozlem,
Yilmaz Ozden Tugba,
Ersoy Ezgi,
Hatipoglu Cakmar Seda Damla,
Goren Ahmet Ceyhan,
Yilmaz Aydogan Hulya,
Ozturk Oguz
Publication year - 2021
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13904
Subject(s) - punica , mcf 7 , traditional medicine , viability assay , antioxidant , cyanidin , chemistry , cell culture , cancer cell , biology , biochemistry , cell , cancer , medicine , human breast , genetics
In this study, eight different pomegranate ( Punica granatum L.) cultivars from Turkey were evaluated for their antioxidant and cytotoxic effects on the MCF‐7 breast cancer cell lines and MCF‐10A breast fibrocystic epithelial cell lines with a focus on their chemical compositions by LC‐MS/MS. Cell lines were treated with pomegranate juice extracts in different doses at selected time intervals (24th, 48th, and 72nd hour). Afterwards, WST‐1 cell proliferation assay was performed to investigate the cytotoxicity of the extracts. Accordingly, all extracts decreased the cell viability of MCF‐7 breast cancer cell lines and had no cytotoxic effect on the cell viability of MCF‐10A cell lines. Among eight extracts, P7 (Izmir 1513), which was rich in anthocyanins such as cyanidin chloride (69.76 ± 8.02 μg/g extract), cyanidin‐3‐O‐glucoside (903.66 ± 101.89 μg/g extract), and punicalagin (992.09 ± 174.53 μg/g extract), was found to demonstrate the strongest cytotoxic activity on MCF‐7 breast cancer cell lines by decreasing the cell viability in half at 24th hour with an IC 50 value of 49.08 µg/ml. Practical applications Eight commercially valuable pomegranate ( Punica granatum ) cultivars from Turkey were examined. Pelargonidin, cyanidin, cyanidin‐3‐O‐gl, callistephin, and delphinidin‐3‐O‐gl were quantified. Two cultivars (P1 and P3) showed comparatively higher antioxidant effects. A cultivar (P7) showed strongest cytotoxic activity against MCF‐7 breast cancer cell line. The cultivars have potential to be used as natural antioxidant and anticancer agents.