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The effect of gamma and electron beam irradiation on the structural and physicochemical properties of myofibrillar protein and myosin from grass carp
Author(s) -
Shi Gangpeng,
Zhou Mingzhu,
Wang Lan,
Xiao Zihao,
Shi Liu,
Jiao Chunhai,
Wu Wenjin,
Li Xin,
Wang Jun,
Qiao Yu,
Liao Li,
Ding Anzi,
Xiong Guangquan
Publication year - 2021
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13828
Subject(s) - chemistry , irradiation , myosin , myofibril , grass carp , sodium dodecyl sulfate , solubility , chromatography , gel electrophoresis , biophysics , biochemistry , organic chemistry , physics , fishery , fish <actinopterygii> , nuclear physics , biology
Myofibrillar protein (MPS) and myosin (MS) from grass carp was irradiated by γ‐ray and electron beam (EB) irradiation with different dose (2, 4, 6, 8, and 10 kGy). The changes in the physicochemical properties (solubility, Ca 2+ ‐ATPase activity, total and reactive sulfhydryl content, surface hydrophobicity [S 0 ‐ANS]), and structure of MPS and MS were investigated in the present work. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analysis revealed that there were degradation and aggregation of MPS and MS caused by irradiation, and the disappearance of myosin heavy chains (MHC) irradiated by EB was earlier than that of irradiated by γ‐ray. As compared with MPS, the extracted MS was more easily destroyed. With the increase of irradiation dose, the particle size, solubility, Ca 2+ ‐ATPase activity, and SH content of MPS and MS decreased ( p < .05), while the S 0 ‐ANS first increased and then decreased. Two‐way analysis of variance results suggested that the degree of protein denaturation depends on the irradiation mode and dose. Compared with γ‐ray irradiation, the EB irradiation had a greater impact on the physicochemical properties of MPS and MS.