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Chlorogenic acid and caffeine combination attenuates adipogenesis by regulating fat metabolism and inhibiting adipocyte differentiation in 3T3‐L1 cells
Author(s) -
Kong Li,
Xu Meng,
Qiu Yangyang,
Liao Mingfu,
Zhang Qingfeng,
Yang Licong,
Zheng Guodong
Publication year - 2021
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13795
Subject(s) - caffeine , adipogenesis , endocrinology , medicine , chemistry , adipocyte , 3t3 l1 , ampk , chlorogenic acid , glut4 , adipose tissue , lipid droplet , biochemistry , biology , glucose transporter , enzyme , insulin , food science , protein kinase a
Obesity is a complex disease spreading in the world. In our previous studies, chlorogenic acid (CGA) and caffeine had ever been reported to reduce the body weight gain and fat accumulation in mice. This study investigated the anti‐obesity effect of CGA and caffeine on 3T3‐L1 cells. According to triglyceride (TG) assay and Oil‐Red O staining, 40 μg/ml CGA and 160 μg/ml caffeine reduced TG content. Moreover, CGA + caffeine inhibited the mRNA expression of major adipogenic markers, PPAR‐γ2 , and C/EBPα in the metaphase and anaphase stages of differentiation induction (Day 2 and 4). CGA + caffeine improved P‐AMPK/AMPK accompanied by decreasing the expression of GPDH and FAS to depress the lipid synthesis, increasing the mRNA expression of ACO and CAT to promote fatty acid oxidation and up‐regulated the expression of hydrolysis‐related enzyme adipose TG lipase (ATGL) and P‐HSL/HSL. Furthermore, CGA + caffeine improved the expression of Glut4 which promoted the glucose transport. Taken together, these data demonstrated CGA + caffeine inhibited 3T3‐L1 cells differentiation in the middle and late stages and reduced the fat accumulation through AMPK pathway by regulating the fat metabolism‐related enzyme in 3T3‐L1 cells to attenuates adipogenesis. Practical applications The aim of this study was to elucidate the potential role of chlorogenic acid and caffeine in the treatment of obesity.