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UPLC‐PDA‐ESI/MS metabolic profiling of dill shoots bioactive fraction; evidence of its antioxidant and hepatoprotective effects in vitro and in vivo
Author(s) -
Rasheed Dalia M.,
Emad Ayat M.,
Ali Sherifa F.,
Ali Sameh S.,
Farag Mohamed A.,
Meselhy Meselhy R.,
Sattar Essam A.
Publication year - 2021
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13741
Subject(s) - chemistry , antioxidant , chromatography , acetaminophen , metabolite , spin trapping , in vivo , glutathione , high performance liquid chromatography , radical , enzyme , biochemistry , microbiology and biotechnology , biology
Hydroxyl radical ( • OH) scavenging capacity of aqueous dill ( Anethum graveolens L.) shoot (ADSh) extract was assessed using electron paramagnetic resonance (EPR) spectroscopy. ADSh extract (at concentrations of 0.5 and 10 mg/ml) exerted high (OH) radical scavenging power. ADSh extract was further fractionated on Diaion HP‐20 column to yield five fractions. EPR spin‐trapping assay revealed fraction 4 (eluted with 75% aq. MeOH) to possess ( • OH) radical scavenging capacity over a concentration range (0.01–10 mg/ml), whereas fraction 2 (eluted with 25% aq. MeOH) appeared to be pro‐oxidant at concentration 0.01 mg/ml. UPLC‐PDA‐ESI‐MS metabolite profiling of ADSh extract revealed 87 metabolites, of which 64 compounds were identified in fraction 4, the most active fraction. Furthermore, ADSh extract demonstrated a hepatoprotective effect against acetaminophen (APAP)‐induced hepatotoxicity in rats. Pretreatment of rats with ADSh extract (200 mg/kg b.wt) markedly attenuated the increased in the serum hepatic enzyme levels. It also increased free glutathione level and total antioxidant capacity in the serum of treated rats. [Correction added on May 3, 2021, after first online publication: "rates" has been changed to "rats" in the previous sentence.] Additionally, levels of (TNF‐α and IL‐1β) were back to almost normal levels compared to the control group. The above findings suggest that ADSh extract has a protective effect against APAP‐induced liver damage.