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In vitro anti‐inflammatory and antioxidant activities of ZnFe 2 O 4 and CrFe 2 O 4 nanoparticles synthesized using Boswellia carteri resin
Author(s) -
Imraish Amer,
Abu Thiab Tuqa,
AlAwaida Wajdy,
AlAmeer Hamzeh J.,
Bustanji Yasser,
Hammad Hana,
Alsharif Mays,
AlHunaiti Afnan
Publication year - 2021
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13730
Subject(s) - chemistry , nuclear chemistry , antioxidant , nanoparticle , bimetallic strip , nitric oxide , zinc , mtt assay , biochemistry , in vitro , organic chemistry , materials science , nanotechnology , catalysis
The development of plant‐based nano‐materials is considered an eco‐friendly technology because it does not involve hazardous chemicals. In this study, bimetallic ZnFe 2 O 4 and CrFe 2 O 4 nanoparticles were synthesized using an aqueous extract of Boswellia carteri resin. Synthesized ZnFe 2 O 4 and CrFe 2 O 4 nanoparticles were characterized by UV‐Vis spectroscopy, FTIR, XRD, and HR–TEM. The anti‐inflammatory activity was investigated in LPS‐stimulated RAW 264.7 macrophages, whereas antioxidant activity was examined using a Hydrogen Peroxide Scavenging Activity Assay, Nitric Oxide Scavenging Activity Assay, and ABTS Radical Scavenging Assay. ZnFe 2 O 4 and CrFe 2 O 4 nanoparticles demonstrated a moderate scavenger of H 2 O 2 with IC 50 values; 87.528 ± 8 μg/ml and 146.4468 ± 12 μg/ml, respectively. While they exhibited a strong scavenger of NO with IC 50 values; 4.01 ± 0.7 μg/ml and 4.01 ± 0.7μg/ml, respectively. Interestingly, ZnFe 2 O 4 and CrFe 2 O 4 nanoparticles revealed an excellent anti‐inflammatory activity by dose‐dependently suppressing mRNA expressions of IL‐1b, IL‐6, and TNF‐α. Also, ZnFe 2 O 4 and CrFe 2 O 4 nanoparticles suppress the protein expression of TNF‐α. Together, our results proved that phyto‐mediated ZnFe 2 O 4 and CrFe 2 O 4 nanoparticles using Boswellia carteri resin have great potential in biomedical applications such as anti‐inflammatory and antioxidant. Practical applications Our phyto‐synthesized chromium iron oxide bimetallic nanoparticles (NPs) have shown a novel and potent anti‐inflammatory activity, with remarkable biosafety toward tested macrophages. Zinc iron oxide bimetallic NPs exhibited anti‐inflammatory effect with a lesser extent compared to the former, with moderate cytotoxicity against tested macrophages. Both zinc and chromium iron oxide NPs exhibited an equivalent antioxidant activity. Our resin‐capped chromium iron oxide NPs are suggested to be a competing nonsteroidal anti‐inflammatory agent; it is further recommended to establish advanced animal studies to confirm their biosafety, stability, and anti‐inflammatory activity accompanied with the antioxidant activity.