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Purification, structural characterization, and biological activities of degraded polysaccharides from Porphyra yezoensis
Author(s) -
Wang Fan,
Kong LiMin,
Xie YuanYuan,
Wang Chong,
Wang XiaoLing,
Wang YanBo,
Fu LingLin,
Zhou Tao
Publication year - 2021
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13661
Subject(s) - polysaccharide , galactose , rhamnose , porphyra , xylose , mannose , biochemistry , chemistry , sephadex , antioxidant , biology , algae , botany , fermentation , enzyme
The degraded polysaccharides from Porphyra yezoensis (DPPY) prepared using the H 2 O 2 –Vc method under optimized conditions were isolated and purified by DEAE Cellulose‐52, and Sephadex G‐100, providing four pure components, namely, DPPY‐0, DPPY‐0.1, DPPY‐0.3, and DPPY‐0.5. Their relative molecular weights were measured to be 10.8, 10.7, 18.7, and 35.5 kDa, respectively. GC‐MS analysis revealed that all the four fractions were mainly composed of galactose, together with a small portion of glucose, mannose, xylose, and rhamnose. Structural analysis revealed that the purified polysaccharides mainly possess a backbone of (1 → 3)‐β‐D‐galactose (1 → 4)‐3,6‐anhydro‐α‐L‐galactopyranose (G‐A) units and (1 → 3)‐β‐D‐galactose (1 → 4)‐α‐L‐galactose‐6‐sulfate (G‐L6S) units. They were found to promote the proliferation of RAW264.7 macrophages and enhance phagocytosis of the RAW264.7 cells. Antioxidant assays indicated that DPPY‐0.5 possessed the most potent reducing power and free radical scavenging ability among the four purified polysaccharides. High sulfate content and proper molecular weight of these fractions are favorable to their immunomodulatory and antioxidant activities. Practical applications Porphyra yezoensis, common economic red algae widely distributed in East Asian countries, contains a high content of polysaccharides with a variety of biological activities. However, P. yezoensis polysaccharide (PPY) has not been well utilized due to the relatively low biological activities and lack of understanding of its structure‐activity relationship. Thus, it is necessary to improve the bioactivities and elucidate the structure‐activity relationship of this polysaccharide for its practical use. In the present work, four purified fractions (DPPY‐0, DPPY‐0.1, DPPY‐0.3, and DPPY‐0.5) were isolated from the degraded P. yezoensis polysaccharide, and were investigated for their antioxidant and immunoregulatory activities. The results of the present work will lay a foundation for the application of the degraded P. yezoensis polysaccharide in the food industry as a functional food ingredient.