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Alleviation of carbon tetrachloride‐induced hepatocellular damage and oxidative stress in rats by Anabaena oryzae phycocyanin
Author(s) -
Osman Ali,
Salama Ali,
Emam Mahmoud Kamal,
Sitohy Mahmoud
Publication year - 2021
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13562
Subject(s) - phycocyanin , carbon tetrachloride , antioxidant , chemistry , oxidative stress , lipid peroxidation , abts , dpph , food science , ccl4 , biochemistry , pharmacology , biology , cyanobacteria , organic chemistry , genetics , bacteria
Phycocyanin isolated from Anabaena biomass was in‐vitro assayed for its antioxidant activity against DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) and ABTS [2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid)] free radical, revealing maximum activities amounting to 77 and 80% at 1,000 µg/ml and SC 50 values about 96 and 111 µg/ml, respectively. A biological experiment was conducted, involving 40 male Wistar Albino rats, divided into five groups. Group I received only the basal diet as a normal control, while groups II, III, IV, and V were administrated intraperitoneal (IP) injection of a single dose of CCl 4 (50% in corn oil) at 0.5 ml/kg body weight. Subsequently, groups II, III, IV, and V received phycocyanin at 0.0, 25, 50, and 100 mg/kg body weight/day. CCl 4 induced considerable increases ( p < .05) in the levels of serum ALT, AST, urea and creatinine, total lipid, and triglycerides coupled with significant reductions ( p < .05) in serum antioxidant enzymes and some liver histopathological deformations compared to the negative control (group 1). Administration of Anabaena oryzae phycocyanin can counteract these CCl 4 ‐induced changes. Practical applications Phycocyanin isolated from Anabaena has beneficial effects such as the antioxidant, antibacterial, anticancer, and hepatoprotective effect. Phycocyanin may play a key role in alleviating oxidative stress, artificially induced by carbon tetrachloride in Albino rats, to ultimately determine its capacity to serve as a natural antioxidant for food and health applications.