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Licorice extract suppresses adipogenesis through regulation of mitotic clonal expansion and adenosine monophosphate‐activated protein kinase in 3T3‐L1 cells
Author(s) -
Lee MunHoe,
Kim HyeongMin,
Chung HeeChul,
Lee JinHee
Publication year - 2020
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13528
Subject(s) - adipogenesis , ampk , protein kinase a , microbiology and biotechnology , biology , kinase , cyclic adenosine monophosphate , cyclin , cell cycle , chemistry , biochemistry , cell , mesenchymal stem cell , receptor
Licorice, the root of Glycyrrhiza glabra , has been observed to possess an anti‐obesity effect. Previous research has suggested that licorice acetone extract (LE) has an influence on mitotic clonal expansion (MCE) and adenosine monophosphate‐activated protein kinase (AMPK), which play a key role in regulating adipogenesis. This study sought further insight into the molecular mechanism of LE’s anti‐obesity effect using 3T3‐L1 adipocytes in vitro. LE inhibited 3T3‐L1 adipogenesis, and the inhibitory effect of LE on adipogenesis was most significant in the early stage of adipogenic differentiation. LE inhibited the protein expression of cyclins and cyclin‐dependent kinases in the MCE stage and arrested cells in the G1 phase of the cell cycle. Furthermore, it activated AMPK via phosphorylation. Moreover, the expression levels of lipid metabolism‐related genes were regulated by LE. These findings suggest the anti‐obesity effect of LE via MCE and AMPK regulation. Practical applications Although the anti‐obesity effects of licorice have been studied, the application of functional food‐related anti‐obesity effects of licorice has been less than that of other extracts. The present study increases the reliability of the anti‐obesity effect of licorice by suggesting a new mechanism of action and expands the application of functional foods related to the anti‐obesity effect of licorice. A new mechanistic insight will not only improve the scientific knowledge but will also help to predict the side effects of licorice's anti‐obesity application.