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Tetrandrine inhibits cell migration and invasion in human nasopharyngeal carcinoma NPC‐TW 039 cells through inhibiting MAPK and RhoA signaling pathways
Author(s) -
Wu ShinHwar,
Chueh FuShin,
Chou YuCheng,
Ma YiShih,
Peng ShuFen,
Lin ChinChung,
Liao ChingLung,
Chen PoYuan,
Hsia TeChun,
Lien JinCherng
Publication year - 2020
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13387
Subject(s) - medicine , nasopharyngeal carcinoma , china , general hospital , rhoa , christian ministry , traditional chinese medicine , family medicine , traditional medicine , alternative medicine , pathology , political science , biology , signal transduction , radiation therapy , biochemistry , law
The objective of this study was to investigate the effects of tetrandrine (TET) on cell migration and invasion of nasopharyngeal carcinoma NPC‐TW 039 cells in vitro. TET at 1–10 μM did not change cell morphology and also did not decrease the total cell viability and proliferation in NPC‐TW 039 cells. It decreased the cell mobility based on decreased wound closure in NPC‐TW 039 cells by wound healing assay. TET suppressed the cell migration and invasion using transwell system. TET reduced MMP‐2 activities at 1–10 μM and these effects are in dose‐dependently. After exposed to various treatments, TET decreased the levels of p‐ERK, p‐JNK, p‐p38, RhoA, and NF‐κB at 48 hr. Based on these findings, we may suggest TET‐inhibited cell migration and invasion of NPC‐TW 039 cells via the suppression of MAPK and RhoA signaling pathways for inhibiting the MMP‐2 and ‐9 expression in vitro. Practical applications Tetrandrine (TET), a bis‐benzylisoquinoline alkaloid, is obtained from the dried root of Stephania tetrandra . TET has been shown to induce cancer cell apoptosis on human cancer cells but its anti‐metastasis effect on cell migration and invasion of nasopharyngeal carcinoma cells has not been investigated. Our results showed that TET significantly repressed the cell mobility, migration, and invasion of NPC‐TW 039 cells in vitro that involved in inhibiting RhoA, Ras accompanying with p38/MAPK signaling pathway. We conclude that TET may be the anticancer agents for nasopharyngeal carcinoma therapy in the future.