Premium
HPLC determination of massoia lactone in fermented Cordyceps sinensis mycelium Cs‐4 and its anticancer activity in vitro
Author(s) -
Sang Qingni,
Pan Yu,
Jiang Zhihong,
Wang Yuerong,
Zhang Hongyang,
Hu Ping
Publication year - 2020
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13336
Subject(s) - mycelium , lactone , fermentation , high performance liquid chromatography , chemistry , cordyceps , mtt assay , chromatography , food science , biochemistry , in vitro , biology , botany
The fermentation product of Cordyceps sinensis mycelium Cs‐4 was commonly used as alternative substitutes of natural C. sinensis . Massoia lactone is the dominant component in the volatile oil of Cs‐4 mycelium. In this research, we present a high performance liquid chromatography (HPLC) method for the quantitation of massoia lactone in Cs‐4 mycelium. The high and stable contents of massoia lactone with values of 2.98–3.77 mg/g, indicated that massoia lactone could be considered as a marker for the quality assessment of this product. The results of MTT and CCK‐8 assay showed that Cs‐4 mycelium volatiles exhibited cytotoxicity against eight malignant tumor cells (IC 50 = 6.0–49.8 μg/ml) in comparison to the anticancer drug 5‐fluorouracil (IC 50 = 17.0–425.3 μg/ml), and massoia lactone might be the chemical basis for the anticancer effects of Cs‐4 mycelium. Compared to the commercial drugs paclitaxel and docetaxel (IC 50 = 253–1973 μg/ml), the Cs‐4 mycelium volatiles and massoia lactone were discovered to possess inhibitory to taxol‐resistant cell lines (IC 50 = 1.5–8.6 μg/ml). Practical applications Considering that there is still a lack of marker components distinctive to Cs‐4 mycelium, the HPLC method represents a useful tool for the quality evaluation of Cs‐4 mycelium. Moreover, the volatile oil of Cs‐4 mycelium and massoia lactone have prominent anticancer property in vitro. It gives a clue that Cs‐4 mycelium, the volatile oil and massoia lactone could be potentially employed in the food and medical industries for its anticancer applications.