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Chlorogenic acid‐enriched extract of Ilex kudingcha C.J. Tseng tea inhibits neutrophil recruitment in injured zebrafish by promoting reverse migration via the focal adhesion pathway
Author(s) -
Zhang Weihao,
Liu Xiande,
Piao Linghua
Publication year - 2020
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13279
Subject(s) - zebrafish , focal adhesion , chlorogenic acid , inflammation , chemistry , pharmacology , protein kinase b , cancer research , microbiology and biotechnology , biology , biochemistry , signal transduction , immunology , gene , food science
Neutrophil‐regulated inflammation plays crucial roles in tissue damage and repair. Dysregulation of the neutrophil response system can contribute to diseases such as cancer. Clearance of excessive neutrophils at the site of inflammation by reverse migration provides a promising strategy to mitigate the negative effects. Chlorogenic acid treatment of injured zebrafish embryos showed low‐developmental toxicity. Using a transgenic zebrafish Tg (mpx: egfp) model, chlorogenic acid‐enriched kudingcha extract promoted neutrophil reverse migration via phosphorylation of ERK and AKT. Using i‐TRAQ analysis, differentially expressed proteins involved in focal adhesion were identified, such as: Cdc42, SRC, MLC, ITGA, and Calpain. In support of this, ERK and AKT proteins are involved in the focal adhesion pathway. Real time qPCR determined that CGA downregulates genes associated with cancer metastasis, such as: HSPA5, YWHAZ, RP17, and ITGAV. Together, these results suggest that CGA‐enriched Kudingcha extract may have potential as an anticancer or anti‐inflammatory therapeutic agent. Practical applications Ilex kudingcha C.J Tseng, commonly referred to as the large‐leaved kudingcha, is a tea variety naturally rich in chlorogenic acid. Chlorogenic acid, the ester of caffeic and quinic acids, has antioxidant, antibacterial, anticancer, and anti‐inflammatory, activities. Kudingcha has several known biological functions, including: anticancer, anti‐inflammatory, antidiabetic, and hypolipidemic effects. Treatment with kudingcha extract reduces the recruitment of neutrophils, potentially by inhibiting focal adhesion, and activation of cancer metastasis‐related genes. Importantly, kudingcha extract could be used as an alternative nutritional supplement for anticancer or anti‐inflammation via its ability to suppress neutrophil recruitment.