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Arthrocen, an avocado‐soy unsaponifiable agent, improves acetic acid‐induced colitis in rat by inhibition of NF‐kB signaling pathway
Author(s) -
Goudarzi Ramin,
Partoazar Alireza,
Mumtaz Faiza,
YousefiManesh Hasan,
Abdollahi Alireza,
Dehpour Ahmadreza,
Rashidian Amir
Publication year - 2020
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13244
Subject(s) - myeloperoxidase , colitis , acetic acid , inflammatory bowel disease , inflammation , pharmacology , tumor necrosis factor alpha , chemistry , medicine , antioxidant , immunology , biochemistry , disease
The goal of the current study was to evaluate the anti‐inflammatory effect of Arthrocen against acetic acid‐induced colitis in rats. Acute inflammation was produced through intrarectal administration of 2 ml diluted acetic acid (4%) solution. All interventions were carried out for 5 days after colitis induction. Arthrocen was administered orally at doses of 30, 60, and 120 mg kg −1 day −1 . Then, macroscopic and microscopic studies were performed. Myeloperoxidase (MPO) activity and tumor necrosis factor‐α (TNF‐α) activity were measured by biochemical and ELISA methods, respectively. Immunohistochemistry was done to investigate the expression of pNF‐κB. The results of this study demonstrated that Arthrocen reduced macroscopic and microscopic damage compared to the acetic acid group. Furthermore, Arthrocen decreased the activity of MPO and TNF‐α as well as the protein expression of pNF‐kB in rat colon tissue. The results of the current study revealed the anti‐inflammatory activity of Arthrocen in acetic acid mediated colon inflammation through suppressing the NF‐κB pathway. Practical applications Inflammatory bowel disease (IBD) is an immune‐mediated chronic relapsing disorder affecting the gastrointestinal tract (GIT) characterized by chronic bowel inflammation. A plant‐based dietary supplement containing avocado and soy unsaponifiable extracts in a ratio of 1:2 is known as Arthrocen. Arthrocen can be used as a complementary drug beside current drugs in clinical trials for the treatment of IBD.