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A novel oligosaccharide isolated from Hericium erinaceus and its protection against LPS‐induced Caco‐2 cells via the TLR4/NF‐κB pathway
Author(s) -
Wang Dandan,
Xu Duoduo,
Zhang Yanqiu,
Zhao Daqing,
Wang Mingxing
Publication year - 2020
Publication title -
journal of food biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 47
eISSN - 1745-4514
pISSN - 0145-8884
DOI - 10.1111/jfbc.13135
Subject(s) - hericium erinaceus , oligosaccharide , mannose , chemistry , monosaccharide , galactose , tlr4 , polysaccharide , hmgb1 , acetic acid , biochemistry , mushroom , caco 2 , residue (chemistry) , in vitro , signal transduction , receptor , food science , raw material , organic chemistry
Abstract A novel oligosaccharide showed that protection against LPS‐induced Caco‐2 cells was purified from the mycelium of Hericium erinaceus (HE). WEP‐1 is mainly composed of neutral monosaccharides with molecular weight of 4,010 Da and of mannose, glucose, and galactose in a molar ratio of 1.2:16.9:1. The structure of WEP‐1 includes α‐D‐Glc (1 → 3) and β‐D‐Gal (1 → 3) as the backbone with β‐D‐Glc (1 → 3) as branches attached to the C‐4 position and β‐D‐Man as a terminal residue. The oligosaccharide reduced acetic acid‐induced colonic mucosa injury in rats. It also showed significant protection against LPS‐induced Caco‐2 cells via the TLR4/NF‐κB pathway. Practical applications In the study, the oligosaccharide from HE has the potential to be developed into functional foods or medicines for the treatment of intestinal diseases. The protection against LPS‐induced Caco‐2 cells via the TLR4/NF‐κB pathway may be a key target for the pharmacological activity of HE .